Hoechst concentration
NettetRecommended conditions for staining cells with Hoechst stains. Cell Type Hoechst Dye Concentration Incubation Conditions Bacteria 0.1 to 12 µg/mL 10 to 30 minutes Live … NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often …
Hoechst concentration
Did you know?
Nettet20. des. 2024 · Spin-labeled BmrCD mutants were concentrated to 70–100 µM using Amicon Ultra-100 centrifugal filters (Millipore) and incubated with nucleotides or Hoechst-33342 (Thermo Fisher). NettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1). Fig.1. Hoechst 33342 (in blue) binding dsDNA. Hoechst 33342 Excitation / Emission Spectra
NettetFluorometry allows specific and sensitive measurement of DNA concentration by use of a fluorescent dye; with common dyes including Hoechst dyes and PicoGreen. Hoechst … Nettet25. sep. 2024 · Hoechst typifies a chemical biology probe with remarkable characteristics, possessing good permeability, high affinity (K d ∼1–10 nM) and specificity, and a fluorescent turn-on of ∼30- to 40-fold upon DNA binding with a large 100 nm Stokes shift, 16 when they bind to adenine-rich regions in the minor groove of DNA (Figure 1a). 17 …
NettetHoechst AG ( German pronunciation: [ˈhøːkst]) was a German chemicals then life-sciences company that became Aventis Deutschland after its merger with France's … NettetA concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. A green fluorescence of unbound Hoechst dye may be observed …
NettetHoechst 33342 (2'- [4-ethoxyphenyl]-5- [4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet …
NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, … memothillNettetHoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced Salt Solution, Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).DMEM+ (Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).. Propidium Iodide … memotech maintenanceNettetHoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 10 6 cells, incubate, and analyze. Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. memotong rasterNettetVolume of Water needed to reconstitute specific mass of Hoechst 33342 *20 mM solution in water* to given concentration. Note that volume is only for preparing stock … memovoc correctionNettetFind hoechst 33342 and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced Search. Structure Search. Search Within. Products Technical Documents Site Content Papers Genes Chromatograms. Shipping. Ships Today (1) Product Category. dyes (4) Brand. Sigma-Aldrich (6) … memotyiouNettet7. okt. 2024 · Hoechst 33342 solution (10 mg/mL in distilled water). The solution is diluted at a concentration of 1 mg/mL with distilled water, filter-sterilized and then stored at −20 °C in 1 mL aliquots in the dark. The “working” stock solution is covered with aluminum foil and kept at 4 °C. 7. memotong foto 4x6NettetBiotium offers stable concentrated stock solutions of Hoechst in water at 10 mg/mL (see ordering information). DAPI – Preferred for Fixed Cell Staining DAPI is somewhat less … memothemafioso - 48 bars