Ph-ncas9-ppe-v2
Webb15 apr. 2024 · To construct the pH-nCas9 (H840A) vector for the control group, the M-MLV sequence of pH-nCas9-PPE was removed using a ClonExpress II Cloning Kit. The PE3 … Webb24 mars 2024 · We used the ePPE to generate rice plants tolerant to sulfonylurea and imidazolinone herbicides, observing an editing frequency of 11.3% compared with 2.1% …
Ph-ncas9-ppe-v2
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Webb22 juni 2024 · Answer. The dCas9, also known as endonuclease deficient Cas9, is a mutant form of Cas9, whose endonuclease activity is removed by mutating the endonuclease … Webb24 mars 2024 · PPE-NC-v1 showed the highest editing efficiencies, ranging from 0.2% to 19.5% in both rice and wheat protoplasts, followed by PPE-NC-v2 and PPE-∆RNase H (Fig. 1f,g ). These three new PPEs...
WebbAbout Caixia Gao. Prof. CaixiaGao Principal Investigator, Ph.D. Research interests: Developing novel technologies to achieve efficient and specific genome engineering, and … Webb24 mars 2024 · Engineered RTs, fusing functional elements or peptides to nCas9-RT (Cas9 nickase-reverse transcriptase), increasing the binding affinity of the nuclear localization signal sequences of nCas9...
WebbPlasmid pH-nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 … WebbTotal vector size (bp) 6388 Vector type Plant Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Spectinomycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name BsaI:AATG:Cas9_2:GCTT:BsaI Species Synthetic Insert Size (bp) 4141 Tag / Fusion Protein
Webb24 juli 2024 · Product Name : pH-nCas9-PPE-V2 article : bacterial resistance : Kanamycin cloning : backbone pHUE411 (modified from pCambia) backbone_mutation …
WebbpH-nCas9-PBE质粒价格1200元,pH-nCas9-PBE(爱迪基因 98163 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为卡那霉素。 pH-nCas9-PBE质粒是植物表达;水稻。 dr patel my chartWebb杭州宝赛生物是专业的质粒菌株公司,承接质粒进口,质粒构建,质粒改造,菌株基因编辑,菌株改造,合成生物学菌株构建服务。宝赛生物提供的质粒菌株产品,都有亲自实用经验提供,100%保证售后质量。宝赛生物可以提供 美国atcc,德国dsmz,美国nrrl,日本jcm,nbrc,英国ncimb,ncyc,荷兰cbs,瑞典 ... dr. patel-nephrologist in merritt islandhttp://enpcce.genetics.cas.cn/PN/CXG/ACXG/ dr patel michigan avenue dearborn miWebbPlasmid nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 … dr patel murfreesboro tnWebbModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites … dr patel nephrologist wichita fallsWebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … dr patel national spine and painWebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, … dr patel nephrology lubbock tx